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KMID : 1161520160200060384
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Animal Cells and Systems
2016 Volume.20 No. 6 p.384 ~ p.393
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The association of haplotypes in IGFBP-3 gene promoter region and tissue expressions in three pig breeds
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Wu Qingyan
Yu Hao
Fang Xibi
Cheng Yunyun
Dong Lijie
Wei Wenzhen
Wang Gang
Fu Haoyu
Liu Songcai
Hao Linlin
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Abstract
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Bama Xiang pig (BM) and Tibetan mini-pig (TM) are used as experimental animals in China; however, the dwarf molecular mechanisms of these Chinese local pig breeds are unknown. IGFBP-3 affects animal growth, carcass and meat quality. The aim of this study was to identify the polymorphisms in the promoter of the IGFBP-3 and analyse their effect on the IGFBP-3 mRNA expression level in liver and muscle tissues. High-density single-nucleotide polymorphisms (SNPs) (31) and InDels (5) were detected in the promoter of the IGFBP-3 in the BM, TM and Junmu No. 1 White (JM, control) pig breeds from 114 individuals by re-sequencing. A perfect Linkage disequilibrium consisted of 13 SNPs was observed in the promoter region and 2 main haplotypes were identified, of which the h1 genotype (GCA-ATGTACATAT) was more prevalent in JM breed than in TM or BM breeds (P?
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KEYWORD
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IGFBP-3, polymorphisms, haplotype, body size, tissue expression
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